Video credit: Ahmed Shaheen (Egypt)
Review: Abdel Rahman El Gamal (founder of the site and channel)
This video was filmed in a shrimp hatchery in Vietnam.
The artificial inseminating in shrimp implies the mating of selected individuals for mating and hence obtaining a regulated supply of fertilized eggs as required for the shrimp farming industry as well as for various genetic programs.
The success of the artificial insemination and the subsequent high rate of fertilization depend on the proper selection of brood shrimp to ensure that females should have full ovarian development, while males should have full and healthy spermatophores.
As shown in the video, male spermatophores are manually ejected by applying gentle pressure to the base of the outer corner of the spermatophore until it slips out of the genital pore. The ripe shrimp female is carefully held so that her thelycum is exposed (not shown). The first spermatophore is placed and secured anterior to the thelycum between the base of the third and fourth pereopods while the second spermatophore is placed and secured posterior to the thelycum between the base of the fourth and fifth pereopods. The glutinous material surrounding the spermatophore structure are spread using an index finger to cover the thelycum. The whole insemination process should be completed very fast to reduce possible handling stress to the female.
The spawning is usually imminent within about 1 to about 24 hours after the insemination conditioned to placing shrimp females in spawning tanks of seawater with optimum salinity 25-36 g/l and temperature (26° to about 30° C).