This lecture was delivered during November, 2014 in Fish Culture Development training course. This course is annually organized by the “Egyptian International Centre for Agriculture – EICA”. This lecture focuses on handling issues during main practices of aquaculture whether in hatcheries or farms. The handling stress is highlighted as well as means to alleviate the stress. The aquaculture practices include stocking, sampling, harvesting, grading, marking, anesthesia, hatchery operations, and transportation.